Total chemical synthesis and X-ray structure of kaliotoxin by racemic protein crystallography
By Brad L Pentelute, Kalyaneswar Mandal, Zachary P Gates, Michael R Sawaya, Todd O Yeates, and Steve Kent
Published in Chemical communications (Cambridge, England) 46(43): 8174-6 on Nov. 21, 2010.
PMID: 20877851. Link to Pubmed page.
Core Facility: Membrane Protein Expression/Purification
Here we report the total synthesis of kaliotoxin by ‘one pot’ native chemical ligation of three synthetic peptides. A racemic mixture of D- and L-kaliotoxin synthetic protein molecules gave crystals in the centrosymmetric space group P1 that diffracted to atomic-resolution (0.95 Å), enabling the X-ray structure of kaliotoxin to be determined by direct methods.