The recent integration of new developments in electron microscopes, direct electron detection cameras, and advances in image analysis methods are allowing the expansion of high resolution structural molecular biology in new and exciting directions by direct visualization of macromolecules and their complexes. The next decades will be dominated by the study of protein-protein, protein-nucleic acid complexes, molecular machines, and their conformational changes in ways that were impossible before due to their size and/or the need to study them in crystalline form. In addition, developments in many aspects of electron microscopy are providing new tools for the study of biological molecules from the single molecule to the cellular level. The inaugural workshop on Advancement and Challenges of Biological CryoEM will address key developments in this fast advancing field, provide an opportunity to discuss the state of the field and exchange views on advancement and challenges of biological cryo-electron microscopy, and foster the research collaboration of biological and biomedical sciences, particularly in Hong Kong, China, and Asia. The workshop will be hold the day after the GRC Conference on three-dimension electron microscopy in Hong Kong, which is the premier cryoEM meeting, to encourage the participation of GRC attendees.